Given the escalating issue of resistant bacteria to antibiotics in healthcare facilities, the practice of isolating patients will continue to be crucial and its significance will only intensify as a vital measure to curb the spread of multidrug-resistant organisms (MDRO) within these institutions. A comprehensive literature analysis was undertaken using electronic databases, with the objective of highlighting the significance of isolation precautions and their influence on the mental well-being of patients. Research has indicated that isolation can have adverse effects on the psychological well-being, safety, satisfaction, and overall care of patients. However, further well-established methods and extensive studies are required to further investigate this matter. Providing patients with information during isolation is an essential element in the effort to alleviate anxiety and suffering.