Bacillus subtilis and other Bacilli are commonly utilized as biological control agents to combat plant bacterial infections, however the specific processes via which they provide protection remain unclear. The aim of our work was to extract B. subtilis strains with high biocontrol efficiency from natural habitats and to understand the mechanisms via which these strains provide plant protection. 4PHCISA25 shown superior biofilm removal capabilities compared to 4PHSA25 against MRSA strain biofilms, as indicated by biofilm removing tests. Mouse tests showed that injecting 4PHCISA25 was safe and effectively prevented abscess formation caused by MRSA within 24 hours, while also aiding in the recovery from clinical symptoms. This study emphasizes the utilization of phages to battle MRSA.