As the years are passing continuously the human being are facing the stringent problem of fossil fuel. Human are also suffering with the problem of emissions that is happening due to the use of petroleum-based fuels. So, it becomes the prime requirement of human to find the such a source of energy that can replace the diesel fuel which should be renewable in nature. In this study a hybrid methyl ester of Calophyllum inophyllum and Waste cooking oil is considered with addition of nano additive Al2O3. The role of additive is to boost up the performance parameters of fuel. For the testing of this hybrid methyl ester the 1C 4S diesel engine is consider with CR of 18. The blends are considered as B20F2, B20F4, B20F6, B20F8, B20F10. The readings are taken in variation of load 0kg, 3kg, 6kg, 9kg, 12kg. The result and discussion indicate that the hybrid methyl ester of B20F8 of Undi+WCO have good result like or more than that of diesel fuel. So proudly it can be said that in future if land area of biodiesel increases then it can replace the diesel fuel in future.