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ISSN 2063-5346
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An Advanced Biological Matrix Extraction method development and Validation for Dacomotinib in Human Plasma (K2 EDTA plasma) by using LC-MS/MS

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Mahesh.M1*, N.Devanna2
» doi: 10.48047/ecb/2023.12.5.167

Abstract

In the current investigation, a validated LC-MS/MS assay was created in order to measure Dacomitinib in K2EDTA human plasma. Dacomitinib was resolved chromatographically using a C18 column and an isocratic mobile phase. With a mean recovery of 82.08% and a linear calibration curve spanning from 0.503 ng/ml to 250.010 ng/ml, the validated procedure meets all of the acceptance criteria by using QuEChERs extraction method. Dacomotinib D10 is the internal standard, and its corresponding mean recovery is 77.45%. With a retention time of 1.35 min for dacomotinib and dacomotinib D10 and a run time of 4 mins, column oven temperature of 30˚ C at a flow rate of 0.800 ml/min, and autosampler temperature of 5˚ C, the method is fully validated and is successful, sensitive, simple, and robust and apply for routine analysis. Dacomitinib's obtained r2 is 0.9992, and the LLOQ is 0.506 ng/ml.

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