Volume - 13 | Issue-1
Volume - 13 | Issue-1
Volume - 13 | Issue-1
Volume - 13 | Issue-1
Volume - 13 | Issue-1
In the current investigation, a validated LC-MS/MS assay was created in order to measure Dacomitinib in K2EDTA human plasma. Dacomitinib was resolved chromatographically using a C18 column and an isocratic mobile phase. With a mean recovery of 82.08% and a linear calibration curve spanning from 0.503 ng/ml to 250.010 ng/ml, the validated procedure meets all of the acceptance criteria by using QuEChERs extraction method. Dacomotinib D10 is the internal standard, and its corresponding mean recovery is 77.45%. With a retention time of 1.35 min for dacomotinib and dacomotinib D10 and a run time of 4 mins, column oven temperature of 30˚ C at a flow rate of 0.800 ml/min, and autosampler temperature of 5˚ C, the method is fully validated and is successful, sensitive, simple, and robust and apply for routine analysis. Dacomitinib's obtained r2 is 0.9992, and the LLOQ is 0.506 ng/ml.