Purpose: Evaluation of accuracy of linear measurements of teeth on CBCT images for application on dental age estimation. Material and Methods: CBCT radiographs of 1000 teeth of patients which range from 16 to 58 years were analyzed to estimate the chronological age by developing regression equation using different linear measurements on tooth. Results: The coefficient of determination of the regression equation derived from the linear ratios was 0.558 and from the linear measurments was 0.338. Conclusion: The prediction equations derived from linear ratios had higher prediction ability than equations derived from linear measurements.