Energy utilization and energy saving is always focused topic for any nation. In this paper, first of all, energy scenario after and before covid-19 is studied. Energy scenario is explaining the vast scope of energy development in the field of bio-fuels, which is the main objective of my research project. Also, as per energy scenario, CO2 emissions produced counts India as a leading nation. This effect can be minimized with the help of biofuel/biodiesel preparation and use in vehicles. In this paper, classification of bio-fuels as first generation, a second generation and third generation is studied and reviewed. Main focus of this paper is on the generation of fuel from non-edible seeds of plants and its availability for the feedstock needed in blend preparation. Bio-diesel produced from edible-oil/non-edible oil/animal waste/plant waste also studied. List of feedstock or raw material available for bio-diesel is reviewed. Meaning of blend, blend preparation methods, engine setup requirements, engine testing methods, result findings and their conclusion is studied. Benefits of bio-diesel, possible ways to use bio-diesel and future scope through development of bio-diesel is studied for further research work.