Quality of care at the health facilities during childbirth remains a keyconcern. Enhanced quality could have the utmost dividend in saving maternal and newbornlives.India continues to intensify its efforts to reduce maternal and neonatal mortality, through the initiatives launched under the umbrella of the National Rural Health Mission. Objective: The objective of this study was to assess the quality in labor rooms against recommended model labor room quality standards in different levels of health facilities in Darjeeling district, West Bengal, India. Methods:This study was a Descriptive epidemiological study with one baseline and a follow up visitconducted inlabor rooms in different levels of health facilities in Darjeeling district, West Bengal. Study was conducted fromMay 2016 to October 2018. Pre-designed and pre-tested semi-structured schedule based on Government of India recommendations for standardization of labor rooms was used. Template was generated in MS excel sheet and analysis was done on SPSS software.Results:Out of the 38 public health facilities there were one medical college, 2 district hospital, two sub-division hospital and 12 Block Primary Health Centre and 2 PHC were included as study unit. among L1 facilitiesmaximum improvements occurred in Bagdogra PHC during follow up visit. Among the L2 facilities maximum improvements occurred in Mirik BPHC, Naxalbari Rural Hospital & Bidhan Nagar PHC during follow up visit. Among L3 facilities Siliguri District Hospital & Kalimpong District Hospital scored higher.Conclusions:Most of the key practices were followed but there were lots of structural issues in labor rooms of each facilities.