To get an insight into the physiological realty of biological cells it is necessary to obtain knowledge regarding their electrical make. It is in this view an attempt has been made to study the dielectric property of human erythrocytes suspended in solutions of different tonicity solutions (Isotonic, Hypertonic, and Hypotonic) using the technique of Dielectrophoresis. This technique is concerned with the electrical constitution of the cell and is sensitive to small perturbations in cell physiology. In the present study, Erythrocytes suspended in different prepared solutions are subjected to Non-Uniform Electric Field produced by Pin-Pin Electrode configuration. Dielectrophoretic Collection Rate of Human Erythrocytes was measured at a constant frequency, time, applied voltage, and concentration of cell. The present study reveals that the tonicity of the medium affects the membrane physiology of human erythrocytes which at the cellular level is assessed using the technique of Dielectrophoresis.