The objective of this study was to evaluate the potential hypoglycemic activity of alcoholic extracts obtained from the flowers of Musa paradisiacal in rats. Additionally, the antidiabetic activity of both alcoholic and aqueous extracts of Musa paradisiaca flowers was investigated in streptozotocin-induced diabetic rats. During preliminary phytochemical screening, glycosides, fixed oils, tannins, phytosterols, and phenolic compounds were identified in the alcoholic extracts. However, the significant effects of the alcoholic extract were observed only at higher doses. In the case of streptozotocin-induced diabetic rats, the alcoholic extract of Musa paradisiaca flowers demonstrated significant antidiabetic activity, particularly at higher doses, surpassing the effects of the standard drug Glibenclamide. Morphological studies involving water consumption, food intake, and body weight indicated similar effects of the alcoholic extracts and Glibenclamide in diabetic rats when administered at higher doses. Furthermore, the histopathological study revealed a notable restoration of damaged cells in the islets of Langerhans, as compared to the effects of glibenclamide. Based on findings, it can be concluded that alcoholic extract of Musa paradisiaca flowers exhibits promising antidiabetic properties, supporting its traditional usage in managing diabetes.