The study aimed to explore the level of job satisfaction among healthcare professionals in the Saudi healthcare sector regarding their professional performance and to determine the relationship between professional experience and job satisfaction. The descriptive survey method was used to achieve the study objectives, with the current population including all healthcare professionals in the government sector in the Kingdom of Saudi Arabia during the academic year 2022. The study had 346 participants, and a questionnaire was used to collect data. The results showed statistically significant differences in the level of job satisfaction among healthcare professionals in the Saudi healthcare sector, specifically regarding the level of professional experience, where differences favored those with professional experience ranging from 11-15 years. Based on the results, the study recommends organizing training courses and educational lectures for healthcare professionals in government healthcare institutions, delivered by experts in dealing with the occupational psychological problems they face. The study also encourages further research on the level of job satisfaction among healthcare professionals in other work environments, such as the private sector, through comparative studies. It also calls for further studies to identify the obstacles affecting the improvement of job satisfaction among healthcare professionals in the healthcare environment and to provide suggestions and mechanisms that would enhance their job satisfaction in the Saudi healthcare sector.