High Performance Liquid Chromatography (HPLC) was used in this study to establish precise robust and Stability Indicating method. Reversed-phase HPLC (RPHPLC) with an end-capped Waters symmetry C18 column (250 × 4.6 mm, 5µm) was employed in this work for separation. Acetonitrile and 0.1% acetic acid made up the binary mobile phase (55:45). With a flow rate of 1.0 ml/min, a run time of 25 minutes, and an injection volume of 25 µl, an isocratic program was employed. The sample cooler and column Temperatures were ambient. At 254 nm, the analytes were detected. The developed method revealed that the sensitivity of the method, accuracy, precision, robustness and stability indicating characteristic parameters satisfy the method validation requirements. Keywords: Isoflavones, HPLC, Method development, Method Validation, Sensitivity of the method, Robustness, Precision, Accuracy and Stability Indicating Characteristics.