This research study aims to evaluate the isolation of Ficus carica pectin extraction, characterization and acute toxic studies. Pharmacognostic studies have been investigated on the ripened Ficus carica fruits using several micro-chemical tests. The fruits were washed, dried and extracted with 95% alcohol. The extract was detected for the identification of phytoconstituent pectin. The evaluation studies were conducted as an important prerequisite step for the determination of the physicochemical properties of the pectin. OECD guidelines are followed for acute toxic studies. The isolated pectin was light brown in colour, rough and odourless with characteristic rough fractures. The pectin was confirmed to have pH as slightly neutral, good flow properties, low moisture content, degree of esterification range was in between 55.6% -65.7 % ; the swelling index was found to be 450. The isolated pectin was characterized using NMR, XRD, FTIR and DSC tests which proved the chemical nature of the pectin is in pure form. No significant changes were observed in haematological parameters, behavioural symptoms, signs of mortality and histopathology studies, during acute toxic studies. The evaluation studies, parameters and results revealed that the extract from the Ficus carica fruit is a novel approach to get high yields of isolated pectin. It can be concluding that Ficus carica fruit pectin can be used as natural disintegrant in oral solid dosage form to prepare ODTs.