Protozoan and anaerobic bacterial infections are treated with ornidazole, a member of the 5-nitroimidazole medication class, by a mechanism involving preactivation by reduction of the nitro group and formation of harmful derivatives and radicals. Another member of the antibiotic family, metronidazole, has been theorised to interfere with photosynthesis by removing electrons from ferredoxin, which in turn prevents NADP+ reduction and increases radical and peroxide production. Here, we demonstrate a novel method by which ornidazole limits photosynthesis. Ornidazole inhibits the activity of two Calvin cycle enzymes, triose-phosphate isomerase (TPI) and glyceraldehyde-3-phosphate dehydrogenase, while having a negligible impact on the photosynthetic electron transport and oxygen photoreduction (GAPDH). Ornidazole is a C-nitro molecule that is 5-nitroimidazole with 3- chloro-2-hydroxypropyl and methyl groups in place of the hydrogens at positions 1 and 2, respectively. It is employed to treat anaerobic bacterial infections as well as sensitive protozoal infections. It has a role as an antiprotozoal drug, an antiinfective agent, an antibacterial drug, an antitrichomonal drug, an epitope and an antiamoebic agent. It is a member of imidazoles, a C-nitro compound, a secondary alcohol and an organochlorine compound. The molecular weight of C7H10ClN3O3 is 219.62 g/mol. Pure ornidazole, dosage forms, biological fluids, and pharmaceutical mixes may be investigated utilizing spectrophotometry, chromatography, electro progressive development, and capillary electrophoretic methods. The objective of this project is to supply, summarise, and explain the several analytical approaches that may be used to quantify ornidazole either in the their natural form or in mixture with some other active constituents in formulations and biological matrices. These categories essentially group the determinations: (1) volumetric analysis; (2) optical methods; (3) chromatographic techniques; (4) electro analytical methods; (5) capillary electrophoretic; (6) bioanalytical methods; and (7) chemometric analysis.