Antimicrobial resistance (AMR) is a significant public health issue, as it can lead to prolonged illnesses, increased healthcare costs, and higher mortality rates. It is also a global health security threat, as resistant infections can spread rapidly across borders, making it challenging to control outbreaks and pandemics. To combat AMR, it is essential to promote responsible use of antimicrobial drugs, such as only prescribing them when necessary, and completing the full course of treatment. Urinary tract infections are the most common infectious disease, it is estimated that more than 150 million UTIs in the world reported per year and it bears as economic and medical burden worldwide and about 35% of healthy individuals suffer from symptoms of UTI at some stages in their lives. Multidrug resistance is a significant public health concern worldwide, as it makes the treatment of infectious diseases more challenging, costly, and sometimes impossible. This study aimed to investigate the antimicrobial resistance mechanism towards MDR Klebsiella pneumoniae spp. Whole genome sequencing (WGS) was carried out on most common Gram-negative pathogen Klebsiella spp. through Next Generation Sequencing (NGS) platform by using an instrument Ion Torrent S5 (540 Chip). The draft genome was annotated using RAST tool kit (RASTtk) and assigned a unique genome identifier of 573.52981. Antimicrobial resistance genes were identified using ResFinder, AMRFinderPlus (NCBI) and BV-BRC (PATRIC) Pipeline. WGS analysis detected several antimicrobial resistance genes like ESBL genes, including carbapenems (blaSHV-11, blaTEM-1, ompK36TD, blaOXA-181, blaCTX-M-15, blaSHV-67). Furthermore, quinolone resistance including oqxA/oqxB, gyrA_S83I, parC_S80I, qnrB. In addition to aminoglycoside modifying enzymes genes (rmtF1, aac(6')-Ib, aph(6)-Id, aph(3'')-Ib), trimethoprim (dfrA12), Sulfonamide (sul2), fosfomycin (fosA) resistance genes were identified