Objective: The study’s aim was to assess the tubular penetration depth and fracture resistance of resin-based AH Plus sealer and bio ceramic-based NeoSealer Flo using scanning electron microscopy (SEM) by various obturation techniques. Material and Methods: 100 recently extracted single-canaled human maxillary upper central incisors were utilized. Samples were prepared and obturated using different obturation techniques and various sealers. For tubular penetration test, a diamond bur with water coolant was used to make root slices with a thickness of 2 mm at 2 to 3, 5 to 6, and 8 to 9 mm from the root tip. Sealer penetration was then assessed by scanning electron microscope at a magnification of 2000x. For fracture resistance test, a universal testing machine was utilized to record the utmost forces responsible for fracture in Newton (N). One way ANOVA test was used to compare results of various groups and sections. Tukey's Post Hoc test was then used for multiple comparisons, with a significance level (P-value) was set as of P-value < 0.05.