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ISSN 2063-5346
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THE EFFECT OF SUPER OXIDE DISMUTASE (SOD) ON THE HEALING OF WHITE RAT CORNEAL EPITHELIAL WOUNDS CORNEA TRAUMA MODEL

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Petty Purwanita, Agung Darmawan, Ratri Prasetya NN, Ramzi Amin
» doi: 10.31838/ecb/2023.12.s3.164

Abstract

Keratitis and corneal erosion are part of eye trauma. Injury to the cornea will cause cell damage, triggering an inflammatory reaction that can increasethe production of ROS (Reactive Oxygen Species). High amounts of ROS can damage cells, modify DNA, destroy proteins and damage mitochondria. SOD (Superoxide Dismutase), one of the endogenous enzymatic antioxidants works by limiting the level of inflammation of the corneal damage caused by oxidation. SOD concentrations were found in the corneal epithelium and endothelium. The aim of this study was to determine the effect of superoxide dismutase concentrations of 2%, 3%, 5% and placebo on the healing of corneal epithelial wound lesions in whiterats with corneal trauma models. Experimental study pre and post-test only with control group design in vivo with 24 samples of white wistar rats that met the inclusion and exclusion criteria. The research was conducted at the Animal House Laboratory, the Biotechnology Laboratory of the UNSRI Medical Faculty and the Eureka Research Center laboratory within 6 months. In this study, 5% SOD showed a smaller reduction in wound area with the largest reduction (mean 0.67cm ± 0.007) followed by SOD 3% (mean 0.67cm ± 0.005). SOD 3% and 5% significantly correlated with weak strength at 48 hours and 72 hours. A significant reduction in the area of corneal epithelial wound lesions was only seen at 48 hours. SOD 5% most affected the healing of corneal epithelial wound lesions, followed by SOD 3%. SOD concentrations of 3% and 5% had a significant correlation on corneal epithelial wound healing at 48 and 72 hours with weak correlation strength.

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