One of the most pressing issues facing modern medicine is the development of treatments for bacterial strains that have developed resistance to existing antibiotics. A total of seven thiadiazolo[20,30]imidazo [4,5- b]quinoxalines and their derivatives were synthesised with the intention of using them as antibacterial agents. The compounds' structures were determined by infrared nuclear magnetic resonance (NMR), mass spectrometry, and elemental analysis. Disc diffusion and agar streak dilution were used to test the newly synthesised compounds for anti-microbial activity against a variety of bacteria. There was a significant inhibitory impact seen in all of the synthetic substances.