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ISSN 2063-5346
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SIMULTANEOUS ESTIMATION OF FIXED DOSAGE COMBINATION OF TELMISARTAN AND BENIDIPINE HYDROCHLORIDE IN HUMAN PLASMA BY HPLC-UV

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Deepak Dalal1*, Ravi Kant2 , Mohit Yadav3
» doi: 10.48047/ecb/2023.12.si5a.0218

Abstract

Objective: Simultaneous estimation of fixed dosage combination of Telmisartan and Benidipine hydrochloride in human plasma by HPLC-UV Methods: The estimation of drugs by RP-HPLC (reverse phase chromatography) was chosen since it is advised for use with ionic and mild to non-polar compounds. Simple, specific, and superior in terms of efficiency, stability, and reproducibility is reverse phase chromatography. For the separation of TEL and BEN, the C18 column 250 x 4.6 mm, 5 µm particle size was chosen. In the mobile phase, various solvent solutions were tested and combined for optimum performance. Telmisartan (40µg/ml) and Benidipine hydrochloride (4 µg/ml) in buffer, pH 4 for Acetonitrile: Water (70:30), a concentration range (8-40 and 0.5-4 µg/ml) as it was displaying excellent peak and a sizable level of resolution. Having the mobile phase run at 1ml/min, photodiode array detectors were used to detect both analytes at 215 nm. The method was validated in different concentration ranges in human plasma. Result: Telmisartan and Benidipine inter and intra-run precision was measured at less than 3.60% and had an accuracy of less than 1.869%. Telmisartan/benidipine in human plasma was validated by linearity, recovery matrix, and stability. The retention time of TEL and BEN is 3.4 minutes and 4.8 minutes in plasma. Conclusion: Suggested method denotes that it is cost-effective and appropriate for the analysis of several samples due to the quick, one-step plasma preparation process and the straightforward HPLC-UV isocratic chromatographic apparatus. combination of drugs performed in human plasma by HPLC and UV method.

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