For the management of Cushing Syndrome, osilodrostat is used. For the estimation of Osilodrostat, a straightforward, accurate, quick, and stability indicating RP-HPLC method was devised. C18 (250 4.6 mm, 5 mm) column and Phos. Buffer pH 3.2- MeOH (40:60) as the mobile phase were used to separate the samples at a flow rate of 1.0 ml/min. The detection process took place at 240 wavelengths. Osilodrostat was discovered to have a retention time of 5.160 minutes. For linearity, accuracy, precision, robustness, LOD, and LOQ, the technique has been validated. Osilodrostat has been found to be linear. Linearity observed for Osilodrostat 10-30 µg/ml. The LOD and LOQ were found 0.0015 µg/ml and 0.048 µg/ml respectively. The estimation of Osilodrostat in API form was successfully implemented using this technique.The RP-HPLC technique was discovered to be straightforward, precise, reliable, and repeatable