It has recently been discovered that there is a correlation between bacteria and carcinogenesis. Fusobacterium nucleatum is frequently detected in oral cancer tissues. Since, studies have only been performed with regard to F. nucleatum in colorectal and oesophageal cancers, we propose to evaluate the role of this F. Nucleatum in the progression of Oral Squamous Cell Carcinoma. Aim: - To identify the role of F. Nucleatum in the progression of oral squamous cell carcinoma. Materials and Methods: -A total number of 14 patients,6 samples from patients with OSCC (Study group),6 normal tissue from patients who have undergone procedures like frenectomy and Crown lengthening (Control group) and 2 SCC samples as a positive control one from oesophagus and another from Sigmoid colon were included in this study. Quantification of F. Nucleatum and expressions like Fad A gene,KRT7 Gene,CCL20 were amplified using Real Time PCR. Statistical analysis: -Statistical analysis of the data was done using SPSS Software version 21.0. Normal distribution was tested by Kolmogorov-Smirnov and Shapiro-Wilks test. Mann–Whitney U test was performed between OSCC patients (study group) and the normal tissues (control group). If the p value obtained was <0.05, it was considered to be statistically significant. Results: -Fusobacterium nucleatum Copy Number was significantly increased in OSCC samples. The mean Fad A, KRT7 and CCL20value was greater in subjects with OSCC compared to Normal subjects. However, the difference was significant statistically only for KRT7 and CCL20. Conclusion: -Fusobacterium species have been suggested to be associated with cell adhesion, tumorigenesis, epithelial mesenchymal transition, inflammasomes, cell cycle, etc. in oral cancer