Introduction: Crude mortality from transmissible infection in India is about 417% per one lakh persons. Organisms causing pus producing infection is alone a leading cause. Unsatisfactory antibiotic management in country like India has maintain to an increase in antibiotic resistant pyogenic infection caused by bacteria in community and hospital settings. Material & Methods: This was an observational and retrospective study from July 2020 to December 2020. A total of 145 isolates from pus samples included. Culture was done onto blood enriched agar and MacConkey agar. After culture all agar plates was placed at 37°C for 24 h. Bacterial confirmation was performed according to standard protocol followed in laboratory. Results: Of the 145 isolates, 51 (35.17%) gram-positive organisms caused pyogenic infections while 94 (64.82%) gram-negative organisms caused pyogenic infections. Staphylococcus aureus was the most common isolate, 33 (22.27%) among gram-positive organisms with 21.7% methicillin-resistant staphylococcus aureus, while Escherichia coli 30 (20.68%) was the most common isolates among the gram-negative organisms. Third generation cephalosporins did not show significant activity against gram-negative microorganisms. Among the Gram-positive infections, Staphylococcus aureus was the predominant organism (74.50%). Among the Gram-negative infections, Escherichia coli was predominant organism (31.91%). Conclusion: Among the Gram-negative microorganisms, 3rd generation cephalosporins show high resistance