This study set out to create a multivariate regression method-based Ultra Violet (UV) spectrophotometric assay that was simple, precise, sensitive, and validated for Pitavastatin analysis. Based on equations created using linear regression analysis and the association between absorbance and concentration at five chosen equidistant wavelengths, this multivariate calibration method was developed. A maximum wavelength of 266 nm was observed for Pitavastatin. For statistical significance testing, the results were examined. The result was a linear plot with a regression coefficient of 0.9993 for the concentration range of 3.5-6.5g/ml. There was a 0.1632 and 0.1759 percent RSD for intra-day and inter-day precision, respectively. The assay result was revealed to be 90%-101%w/w.