Cardiovascular diseases (CVDs) are increasingly the leading cause of mortality worldwide. One naturally occurring plant, Brassica Cretica, is used to address a variety of illnesses, including heart conditions. There is, however, little information available regarding the plant's effectiveness and safety. Objective: To evaluate the prophylactic effects of hydroethanolic extract of Brassica Cretica leaves on cyclophosphamide-induced myocardial injury. Methods: Rats were randomized into 6 groups of 6 rats each, namely Saline control, positive and negative control, hsydroethanolic extract of Brassica cretica (HEBC) in three dose levels. The prophylactic effects of the hydroethanolic extract was evaluated based on anatomical, biochemical and histopathological methods. The parameters such as body weight, cardiac mass to body mass ratio, troponin-I, alanine transaminase (ALT), aspartate aminotransferase (AST), creatine kinase-myoglobin binding (CK-MB), total cholesterol were observed.