The current study aimed to explore the anti-bacterial, anti-oxidant, and anti-bacterial effects of the hydro-methanolic root extract of Glycyrrhiza glabra (Leguminoseaanti-bacterial) using standard screening approaches, disc diffusion, and deoxyribose methods, respectively. Different levels of effect were seen when various study approaches were applied. During phytochemical screening, Glycyrrhiza glabra revealed the presence of secondary metabolites including flavonoids, saponins, glycosides, terpenoids, etc. Moreover, it effectively inhibited virtually all of the test species' microorganisms. With a 10 mm inhibitory zone, it showed the highest degree of sensitivity to Shigella flexineri. compared to the ascorbic acid positive control standard.