To investigate the possible bioactive compounds in the ethanol extracts of whole plants of Henckelia incana and Henckelia humboldtiana. This is doneusing GC-MS methods. GC-MS analysis of the plant extracts were completed by employing a Perkin-Elmer GC Clarus 500 system and mass spectra of the compounds found in the extracts were matched with the data in the library of National Institute of Standards and Technology (NIST). Phytochemical screening of the diverse solvent extracts of the H. incana and H. humboldtiana disclosed the presence of alkaloids, quinones, catechins, flavonoids, phenols, saponins, steroids, tannins, terpenoids, sugars, glycosides and xanthoproteins. In the whole plants studied, forty and thirty five phytocompounds were regulated to be present in the GC-MS analysis. The active principles with their retaining time, molecular formula, molecular weight, peak area and their structure were envisaged. The most prevailing compounds were 6-nonadecyltetrahydro-2H-pyran-2-one, carbonic acid, but-2-yn-1-yl eicosylestaer, octadecane-3-ethyl-5 (2-ethyl butyl); n-hexadecanoic acid, stigmasta-5, 20(22)-dien-3-ol, dotriacontane, propane, 2, 2-diethoxy, propanoic acid, ethyl ester and oleic acid. The occurrence of numerous bioactive compounds justifies the use of this plant for treating various ailments by traditional practitioners.