The present communication attempts to investigate pharmacological, physicochemical analysis and chromatoghraphic profile of ethanol extract of Annona squamosa and Aegle marmelos leaves. This work showed the soxhelt extraction process, phytochemical screening, High Performance Thin Layer Chromatoghraphy and therapeutic importance of the medicinal herbs. Aim and objective: The objective of study is to investigate the preliminary screening of active constituents present in the ethanolic extract of leaves of Annona squamosa and Aegle marmelos. The phytochemicals was extracted separately with distilled water and 96% ethanol by soxhlet extraction method. The present study includes pharmacognostic test detection of alkaloids, glycosides, phenols, carbohydrates, saponins, reducing sugar, flavonoids, tannins. A wide variety of pharmacologically active compounds presented in Annona squamosa leaf extract alkaloids, glycosides, carbohydrates, phenols and saponins. were found to present in the leaves of Annona squamosa and Aegle marmelos. Methods: High performance thin layer chromatography, soxhlet extraction. HPTLC profile of ethanolic extact of herbs had been studied. The generated dada has provided the basis for wide uses as a therapeutic in the traditional and folk medicines. Results: The Rf values of ethanolic extract of Annona squamosa leaf extract run under Chloroform: Methanol(8:2) solvent system were obtained 0.10, 0.21, 0.36, 0.38, 0.42, 0.45, 0.53,0.60, 0.81, 0.86. The Rf values of ethanolic extract of Aegle marmelos leaf extract run under Petrolium Ether : Ethyl Acetate (2:1) solvent system were obtained 0.37,0.44,0.54