Matricaria chamomile is one of the oldest known herbs of traditional medicine and belongs to Asteraceae family. The present study was based on the selection, extraction, purification, and pharmacological evaluation of analgesic action of extracts of Matricaria chamomile in rats. Diclofenac sodium was purchased from Bharat medical store, Bareilly, UP. Alcohol (ethanol and methanol), distilled water, ethyl acetate, n-butanol, petroleum ether was obtained from the local chemical supplier. Clevenger apparatus, rotatory evaporator, and desiccator were used in this study. Flowers and stem from plants- Matricaria chamomilla selected to perform the study. The fresh whole plant of Matricaria chamomilla collected from the local nursery in Bareilly. The plant was identified and authenticated by a Dr. Alok Shrivastava, Professor (Department of plant science), Faculty of sciences, M.J.P. Rohilkhand University