To evaluate the neutrophil engraftment after Hematopoietic Stem Cell Transplantation (HSCT) in a group of pediatric patients diagnosed with non-malignant hematological disorders and to relate the possible effects of different patients’ and transplant variables such as gender, age, conditioning protocol, graft vs host disease (GVHD) etc., on the neutrophils engraftment. Methods: 17 children who had non-malignant hematological disorders and who underwent allogeneic HSCT using peripheral blood stem cells (PBSC) as stem cell source were included. Neutrophils engraftment was defined as the first of three consecutive days in which the peripheral blood absolute neutrophilic count was ≥ 500/mm3. Results: Neutrophils engraftment occurred at a mean of 13.41 ± 3.83 days post-transplant. There was no relation between neutrophil engraftment and gender, original disease, cytomegalovirus (CMV) reactivation, acute GVHD, chronic GVHD, conditioning protocol and GVHD prophylaxis protocol. There were no correlation between the date of neutrophil engraftment post HSCT and age of patients at HSCT, age of donors at HSCT and CD34 count of infused stem cells. Conclusion: When using PBSC as stem cells source, neutrophils engraftment occurred at a mean of 13.41 ± 3.83 days post-transplant, faster than reported with other stem cell sources. The neutrophils engraftment appears not to be affected with possible effects of different patients’ or other transplant variables.