Background: Hepatitis A is a form of viral hepatitis which spread through feco_oral route. It is most common among children and require more attention from health care personal. The Aim of the study: Is to evaluate mothers' awareness regarding hepatitis A virus among children in a rural area. Design: A descriptive correlational research design was used. Setting: This study was conducted at EL Raheb village, ShebinElkom Center, Menofia Governorate. Sample: A multi stage random sample of 315 mothers. Tools: Two tools were used for data collection, tool I: Structured interviewing questionnaire includes four parts; mothers' demographic characteristics, home environmental conditions, mothers' knowledge and attitude regarding hepatitis A virus, tool II: Observational checklist for mothers to assess their practices regarding hepatitis A virus. Results: There were 83.8% of the studied mothers had poor knowledge, 8.3% had average and 7.9% had good level of knowledge regarding hepatitis A virus. Also, 84.5% of the studied mothers had positive attitude while 15.5% of them had negative attitude toward hepatitis A virus. In addition, 83.8% of the studied mothers had adequate level of total practices and 16.2% of them had inadequate level of total practices regarding hepatitis A virus. There were statistically significant relation between demographic characteristics and knowledge, attitude and practices among the studied mothers. Conclusion: The majority of the studied mothers had poor level of knowledge, positive attitude and adequate level of practices regarding hepatitis A virus. Recommendations: Implementing health educational programs should be applied in a large scale at maternal and child health center to improve mothers’ knowledge, attitude and practices regarding hepatitis A virus among children.