Background: The reference values for the sagittal diameter of the cervical spinal canal derived from MRI are still not available in adult population of Uttar Pradesh.The sagittal diameter of the cervical spinal canal is important in traumatic and degenerative conditions of cervical region. The study is done in MRI as the accuracy of MRI is more than the normal plain radiographs. Aim and Objectives: 1. To measure the sagittal diameter of the cervical spinal canal by MRI in both sexes from C3 to C7 vertebrae 2. To provide standard MRI values for the sagittal diameter of the cervical spinal canal with respect to spinal level in male and female. 3. To compare the morphometric data in different age groups. Materials and methods: Magnetic Resonance Imaging (MRI) based on midsagittal diameter of the cervical spinal canal of the lower cervical spine (C3-C7) from 320 subjects (160 males and 160 females) of 18 to 59 years of age. This cross-sectional study was conducted in the Department of Radiology, Santosh Medical College, Ghaziabad Uttar Pradesh in collaboration with Department of Radiology, Hind Institute of Medical Sciences, Barabanki Uttar Pradesh. Results: The sagittal diameter of the spinal canal ranged from 12.08 mm to 18.65 mm in males and 12.01 mm to 17.87 mm in females. The mean values of sagittal diameter of vertebral canal from C3 to C7 are 14.59 mm ± 1.01 in males and 15.26 mm ± 1.11 in females. Conclusions: The sagittal diameter of the cervical spinal canal in general was higher in males as compared to females at all vertebral levels and in all age groups. The sagittal diameter of the spinal canal was narrowest at the level of C4 vertebra in all age group and sexes. Standard MRI values for the sagittal diameter of the cervical spinal canal were established for the adult population of each sex and each age group by this study.