Nanotechnology is an advanced field of research and nanotechnology can be defined as the production of various materials at the magnitude of nanoscale. Nanoparticles are an extensive class of materials that can be classified based on properties, size, and shape. These are very small particles from 1 to 100 nm in the size range. One of the paramount utilization of nanoparticles in biological and biomedical sciences is nanodevices which can be used for drug delivery or compound of reduced size which is currently referred to as “nanomedicine. As nanoparticles have proved to be efficient carriers in drug delivery, currently drug delivery across the blood-brain barrier through nanoparticles is gaining interest. Iron oxide based nanoparticles' magnetic properties have significant potential in the biomedical and clinical fields because of their unique properties. Magnetic nanoparticles have proven to be efficient to cross the blood-brain barrier. In this present research, iron-oxide nanoparticles are synthesized and characterized by infrared spectroscopy, atomic absorption spectroscopy, and field emission scanning electron microscopic studies. PC-12 cell lines were utilized to assess the therapeutic efficiency of synthesized iron oxide nanostructures.