Background: Meditation confers health and longevity, both of which are considerably determined by one’s experiences in terms of stress and emotions. Involved in diverse cellular and physiological functions, hTERT gene relates with health and longevity. The influence of a long adopted meditation “Heartfulness” on hTERT gene expression, perceived stress and emotional wellness has not been reported. The present cross-sectional study aimed to assess the expression of hTERT gene, Perceived stress and Emotional wellness in long-term meditators. Methods: Self-reported healthy, age and gender matched meditators and non-meditators were assessed for hTERT gene expression, perceived stress and emotional wellness. Results: Meditators demonstrated an overall higher hTERT, lesser perceived stress (p<=0.002), greater emotional wellness (p<0.005) and lesser sleep hours (p<0.0001). Significantly higher (p=0.022) hTERT was noted in meditators of >40 years’ age. Duration of daily meditation correlated positively with hTERT expression. Conclusion: Our results are suggestive of a significant healthier aging response along with better mental and emotional levels in the meditators in a real-world environment. Also, duration of daily meditation might play a role in determining health and longevity.