Moringa oleifera is the most widely cultivated species of the moringaceae family, owing to it’s to high nutritional and therapeutic values for mankind. Micro propagation, which is a part of plant tissue culture helps to produce large number of plants population in a short time, in order to obtain genetically uniform plants for propagation. The in vitro nodal bud culture establishment was found to be the maximum at BAP @ 2.0 mg/l and induction of multiple shoots, nodal explants were cultured on Murashige and Skoog (MS) agar medium supplemented with various concentration of 6-Benzyl Adenine Purine (BAP) and maintained at 25±2°C in an incubation chamber. Multiple shoots were observed from the nodal explants cultured on MS medium containing 2.0 mg/l BAP at an average of 4.5 shoots per explant and a mean shoot length of 4.84 cm. For in vitro root induction, the multiple shoots were cultured on ½ MS medium supplemented with IBA 2.0 mg/l which produced the maximum number of roots (5.4 roots per shoot) with the highest mean root length of 6.3 cm. This study describes the micropropagation protocol for mass production of genetically uniform progenies of moringa which can be used in breeding programme.