Azadirachta indica is a well-grown plant abundantly present in Asian countries in various climatic conditions. Aim: The present study evaluation of woung healing activity (In-vitro and In-vivo) of the A. indica methanolic extract silver nanoparticle. Material and Methods: Conventional and GC-MS phytochemical analysis confirms that A. indica methanolic extracts bioactive secondary metabolites. By metal reduction method was used to synthesise silver nanoparticles with the help of A. indica methanolic extract. A. indica methanolic silver NPs studies cytotoxicity and wound healing activity (In-vitro - scratch assay). Transdermal patches were prepared for A. indica methanolic silver NPs by solvent casting method. Wound healing activity (In-vivo) of A. indica methanolic silver NPs transdermal patches conducted in Wister albino rats. Results: Phytochemical analysis of A. indica methanolic extract confirms that it has bioactive secondary metabolites (Copaene, Caryophyllene, Hexanedioic acid, bis(2-methyl propyl) ester, Triacontanoic acid, 2- n-Hexadecanoic acid, Octadecanoic acid, Tetradecanoic acid and 1,4-Eicosadiene etc.). A. indica methanolic silver NPs showing good migration properties (24 hr) with L6 cell lines. F2 formulation A. indica methanolic silver NPs transdermal patch having good drug leasing properties (%). In-vivo wound healing studies revealed that the F2 formulation outperformed the standard drug regarding wound healing properties. The size of the animals' wounds was completely reduced by the 14th day, just like with the standard drug. Conclusion: As a result, A. indica methanolic silver NPs transdermal patches can effectively treat wound healing. Various bioactive phytoconstituents in the developed formulation result in effective wound-healing activity.