In the present study, raw milk sample was collected from dairy farms to isolation and identification of Pseudomonas aeruginosa. The identification of P. aeruginosa was carried out using gram staining, capsule staining and endospore staining. Inhibition of P. aeruginosa was also determined by colony counting method up to 10 days after inoculation on nutrient agar medium supplemented with sodium chloride that used as anti-bacterial agent. In gram staining, pink color of gram-negative bacteria was observed and in capsule staining, bacterial cells and the proteinaceous background appeared purplish while the capsules appeared transparent. Endospore staining of P. aeruginosa was also positive with visualized endospores in contrast to vegetative cells. All the three staining confirmed the P. aeruginosa bacteria. Whereas the result of anti-bacterial method revealed that the growth of P. aeruginosa was completely inhibited at all observation days due to the sodium chloride presence in nutrient agar medium. The present research depicts the significance of NaCl for aseptic purposes and is one of the best natural harmless antiseptic which has major prospects in general public health measures