In human body Chemical speciation shows significant part in comprehension the biological and physiological partsof metal ions and ligands. This study has been revealed that the complexation of Glycylglycine and Histamine with crucial metal ions in Triton X–100 (TX100). The analysis has been carried out using potentiometric method and the outcomesachieved in this examination have been progressed via gran plot method, SCPHD and MINIQUAD75 is utilized for best fit chemical model and refinement of results. In this study effect of surfactant on ternary complexes stability constants, effect of influential parameters like concentration of acid, alkali, ligands and metal ions on the stability constants have been evaluated by conducted an experiment.MLXH2, ML2XH2 and MLX2H3complexes formed in the present study. Chemical problems were correlated by using the advanced principles of mathematics and fuzzy logic, which is called chemo metrics. Errors in correction factor, ligand concentration and metal concentration less on the stability constants of ternary complexes than other parameters like alkali and acids. Influence of surfactant on stability constants of ternary complexes and effect of surfactant on distribution of metal - ligand species were also explained