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ISSN 2063-5346
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DEVELOPMENT AND VALIDATION OF ANALYTICAL METHOD FOR QUANTIFYING ISOFLAVONES

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Swathi K, Rajput Jamatsingh Darbarsingh
» doi: 10.31838/ecb/2023.12.sa1.130

Abstract

Many plant compounds that function as phytoestrogens in mammals are isoflavones, derivatives of the naturally occurring isoflavone. Beans and other members of the Fabaceae family are the only plants that naturally produce isoflavones (Leguminosae). There is little evidence for the safety of long-term supplementation with isoflavones or closely related phytoestrogens, despite the fact that these compounds are sold as dietary supplements and may have health benefits. There may be dangers associated with taking in too many isoflavones, according to some research. For example, there is a lingering fear that breast cancer may be more common in women with a family history of the disease, but good clinical studies have not confirmed this. The study's overarching goal was to establish a protocol for the validation of Isoflavones analysis, with a focus on High Performance Liquid Chromatography (HPLC). Separation was achieved using reversed-phase high-performance liquid chromatography (RP-HPLC) with an endcapped Waters symmetry C18 column (250 4.6 mm, 5 μm). Acetonitrile and 0.1% acetic acid made up the binary mobile phase (55:45). The injection volume was 25μl, the run time was 25 minutes, and the flow rate was 1.0 ml/min in an isocratic programme. At 254 nm, analytes were detected with a Photo-diode array (PDA). System suitability and selectivity parameters were shown to be within acceptable ranges, validating the developed method.

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