To treat bacterial infections, herbs have long been utilized.Numerous pathogenic Escherichia coli strains have been shown to be resistant to frequently administered antibiotics, raising concerns about antibacterial resistance. This study compared the effectiveness of using generic medication preparations to herbal remedies in treating pathogenic E. coli strains that have developed antibiotic resistance. To evaluate the effectiveness of both therapies, the study combined in vitro and in vivo trials.The studies herbal remedy comprised a concoction of extracts from six distinct plant species, each of which is well-known for having antibacterial capabilities. The generic medication mixture employed was a blend of two widely used antibiotics. According to the study, pathogenic E. coli bacteria that were resistant to the generic medication formulation may be effectively stopped from growing by the herbal preparation. In fact, it was discovered that the herbal preparation was more successful at combating antibacterial resistance than the generic medication preparation.In addition to being used in conventional treatment, complementary and alternative therapies from various traditions. In addition to being used in conventional treatment, complementary anumerous strains of pathogenic Escherichia coli are showing resistance to commonly prescribed antibiotics, which is a growing concern for health authorities around the world. Exploring different methods of treating bacterial infections has become urgently necessary as a result of this. Since ancient times, herbal remedies have been used as a safe, all-natural treatment for a wide range of illnesses, including bacterial infections. A wide variety of phytochemicals found in them have been shown to have antibacterial activities. Due to this, herbal remedies are being investigated as a potential substitution for traditional antibiotics in the fight against antibacterial resistance and alternative therapies from various traditions. There have been claims of efficient antibacterial agents in plant materials such as leaves, flowers, stems, roots, and other parts. In our research study we conducted invtiro studies to detect and compare the generic drugs with herbal drugs to explore the antibacterial resistance of Ecoli.