India’s dye industry produces every type of dyes and pigments.Production of dye stuff and pigments in India is close to 80,000 tons.India is the second largest exporter of dye stuffs and intermediates after china .the textile industry account for the largest consumption of dyestuffs.During drying process 5-10% of dyes are released in textile wastewater streams which is ultimately, reach to the receiving natural wastebodies . Textile industries are reported to use the largest amount of dyes (60-70%) compared to other industries. In this present study the isolates obtained from waste water collected from textile industry was characterized morphologically and biochemically. Based on the comparison of biochemical characteristics with standard description the bacterial isolates was identified as Bacillus species and fungal isolates was identified as Aspergillus. The isolate such as bacillus and Aspergillus species exhibit different pH, temperature and inoculums response to the decolorization activity.In pH high level of decolourization was observed in pH6 and pH7. In temperature high amount of decolourization is seen best at 25ºC. In inoculums decolourization high dye decolorization was observed in 500µl. In our present study shown better effect in inoculums, temperature, pH and showed 80% of decolorization in both bacteria and fungi