The aim is to analyze cytotoxic effects Lodhra and cinnamon bark formulation and its assisted zinc oxide nanoparticles Materials and Methods: 200 ml of distilled water was used to dissolve 2g of iodine-free salt. 10–12 ml of saline water were added to 6 well ELISA plates. That was followed by the progressive addition of 10 nauplii (5µL,10µL,20µL,40µL,80µL, and control) to each well. The nanoparticles were then introduced in the appropriate concentrations. For 24 hours, the plates were incubated. The ELISA plates were examined after 24 hours to count the live nauplii that were present and to determine their number using the formula. number of dead nauplii/number of dead nauplii+number of live nauplii×100. Results: Cytotoxic effects of lodhra and cinnamon bark and its mediated ZnO Nanoparticles show an incremental pattern with increase in concentration, where LD 50 concentration was found to be 80µl. Conclusion: With the above results and discussion we come to the conclusion that lodhra and cinnamon bark and its mediated ZnO nanoparticles have potent cytotoxic activity. It’s activity increases with increase in dosage. Further study to be used against a range of human cancer cell lines in cancer Therapy.