The Vernonia cinerea plant is found all throughout India and is a species of the Asteraceae family. Folk medicine uses Vernonia cinerea (L.) as a treatment for a number of ailments. The plant is advised in cases of intermittent fever, filariasis, blisters, boils, etc. by the Indian Ayurvedic Pharmacopoeia. Therefore, the primary goals of the current work are to screen different phytochemicals for antioxidant and antimicrobial activities in various Vernonia cinerea extracts. Vernonia cinerea's phytochemical analysis revealed that its ethanol and chloroform extracts included alkaloids, flavonoids, tannins, terpenoids, glycosides, phenols, saponins, steroids, proteins, and carbohydrates. Alkaloid, Flavonoid, Tannin, Terpenoid, Glycoside, Phenol, and Steroid were all found in hexane extracts. The presence of these substances strongly suggests that V. cinerea has a variety of therapeutic characteristics. The 2, 2-diphenyl-1-picryl-hydrazyl (DPPH), hydroxyl radical, superoxide radical, and ferric reducing antioxidant potential (FRAP) assays were used to measure antioxidant activity, with ascorbic acid serving as the reference antioxidant. It was discovered that the extract effectively scavenges free radicals. Particularly, the ethanol extracts demonstrated more DPPH and hydroxyl radical scavenging inhibition than the standard treatment. Using the agar diffusion technique, antimicrobial activity were evaluated against microorganisms. All of the extracts had a high level of antimicrobial activity against the pathogens that were subjected to the test, with zones of inhibition ranging from 7.0 to 20 mm. The findings of this study supported the traditional medical practice of using V. cinerea to treat a variety of diseases by demonstrating that the plant's solvent extracts have antimicrobial and antioxidant properties