It is suggested that vitamin D deficiency is associated with cardiovascular disease (CVD) via its effect on lipid profiles. The objective of this study was to determine the association between serum levels of vitamin- D and lipid profiles in patients with essential hypertension. Materials and methods: A cross-sectional study was done in the Department of Biochemistry on 200 subjects in the age group of 21-80 years, attended OP Hind Institute of Medical Sciences U.P. Serum 25hydroxyvitamin D levels were estimated by chemiluminescence immunoassay (CLIA) and lipid profiles also (including triglyceride (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL), and total cholesterol) were measured. Data collected included age, gender, vitamin D levels, and lipid levels. The chi-square and independent sample t-tests were applied when appropriate, for comparisons between groups to determine significance. A P-value of less than 0.05 was considered statistically significant. Result: The study included 200 hypertensive patients. Patient ages ranged from 21 to 80 years with a mean age of 56.2 ± 11.8 years. Cholesterol levels were observed to be high among 61 (32.3%) patients. Considering vitamin D, the average level among male patients was 26.526 ng/ml compared to 26 ng/ml% among females (P = 0.742). Conclusion: The results of present study show that serum concentrations of 25(OH) D were inversely associated with lipid profile. More interventional studies are needed to confirm the relationship between serum concentration of vitamin D and lipid profile in patients with essential hypertension.