Antibiotic resistance has emerged as a threat to global health, food security, and development today. Antibiotic resistance can occur naturally but mainly due to misuse or overuse of antibiotics, which results in recalcitrant infections and Antimicrobial Resistance (AMR) among bacterial pathogens. These mainly include the MDR strains (multi-drug resistant) of ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter Baumann, Pseudomonas aeruginosa, and Enterobacter species). These bacterial pathogens have the potential to “escape” antibiotics and other traditional therapies. ESKAPE Pathogens have been placed in the list of 12 bacteria by World Health Organization (WHO), against which development of new antibiotics is vital. In the present study, physico-chemical and phytochemical analysis of various extracts from flower, stem and roots was done. Further, methanolic and ethanolic extracts of Kalanchoe pinnata (Crassulaceae),was screened for antimicrobial activity against ESKAPE Pathogens. The results of the antimicrobial activity showed that the methanol stem extract significantly (P< 0.01) demonstrated antibacterial activities against S. aureus, while the aqueous stem extract significantly (P< 0.01) showed antibacterial activities against P. aeruginosa and E. faecium, both at a concentration of 25 mg/ml and above. Also, the methanol and the aqueous extracts showed the least antibacterial activities at a concentration of 12.5 mg/ml for S. aureus and E. faecium, respectively.For MIC, the antibacterial activities of the methanol extract were significantly (P< 0.01) different from those of the aqueous extract and Gentamicin