The practice of traditional medicine for the control of fertility in most parts of India is based on the uses of plant medicines for many years. Tabernaemontana divaricata (T. divaricata) from Apocynaceae family offers the traditional folklore medicinal benefits such as an anti-epileptic, antifertility, anti-inflammatory, anti-mania, brain tonic, and anti-oxidant. The aim of the present study was to evaluate the antifertility effect of aqueous and ethanolic extract of T. divaricata roots in female Wistar rats. The anti-fertility activity of the extracts was evaluated using five experimental animal models: 1) Estrogenic activity was carried out in immature female rats using ethinyl estradiol as standard. The evaluation parameters include changes in uterine weight and histopathology of uterus. 2) Anti-implantation and early abortifacient activity was performed in female Wistar rats. The number of implants and resorbtions were compared to vehicle control.3) Anti-ovulatory activity 4) Study of estrous cycle. 5) Study of reproductive hormones. In estrogenic activity, the ethanolic and aqueous extracts were offered significant estrogen-like activity at 400 mg/kg, p.o. by increasing the uterine weight compared to vehicle control group. In Anti-implantation and early abortifacient activity study, ethanolic (400 mg/kg, p.o.) showed significant effect and it was evident by decrease in the number of implants and increase in the number of resorbtions compared to vehicle control group. In anti-ovulatory activity the ethanolic extract of T. divaricata at 150 mg/kg and 300 mg/kg had no effect on ovarian weight or cholesterol levels as compared to the vehicle-treated group. In estrous cycle with a significant increase in diestrum phase length and estrus stage extension at the higher dose (450 mg/kg body weight/day). In reproductive hormones study's cytological, hormonal, and reproductive screening, the ethanolic extract of T. divaricata may have an antifertility effect. The above results revealed the potential, reversible female antifertility effect of ethanolic extract of T. divaricata root.