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ISSN 2063-5346
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Analytical Method Development and Validation of Raloxifene Hydrochloride by RP-HPLC

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Kamalandu Roy, R.S. Chandan, Krishnalekha Bandyopadhyay, Anand Kumar Tengli. Manchi Bhargava Sai
» doi: 10.31838/ecb/2023.12.si4.087

Abstract

A precise, rapid, easy and economical RP-HPLC technique for the detection of Raloxifene HCL was developed by using Acetonitrile and Milli-Q water along with 0.1% Ortho Phosphoric Acid (OPA) 50:50 v/v as mobile phase. The maintained flow rate was kept at 0.5mL/minute. At 287nm respectively by using UV detector detection of Raloxifene HCL was performed. RT of Raloxifene HCL was identified at 2.9 min respectively by this proposed method. The R2 value is 0.996, the LOD value is 1.5µg/ml and the LOQ value is 10g/ml. The proposed method is use of the routine Quality control. The R2 value is 0.996, the LOD value is 0.8µg/ml and the LOQ value is 0.5µg/ml. The accuracy was found to be 98.71%.

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