Glaucoma is the condition in which vision loss is caused due to damage to the optical nerve. A study was conducted with an aim to assess the knowledge regarding glaucoma among adults in selected areas of Pune City. Previous researches conducted in other parts of India it is seen that knowledge related to glaucoma is very low, so researchers aims to provide some knowledge related to causes, sign and symptoms, treatment and prevention of glaucoma to the samples participating in the research. Study title: “ A Study to assess the knowledge regarding glaucoma among adults residing in selected areas of Pune City. ” The objective of the study was to assess the knowledge regarding glaucoma and to find association between selected demographic variables. Material and methods: The quantitative research approach was used for this study. The sample size was 200. It included adult from selected areas of Pune City of age 40-60 years. The self structured questionnaire tools used to collect data. The tool was evaluated and validated by 5 experts. The questionnaire consist 16 items and two section (Demographic section & knowledge section). Result: Majority 62.5% are from 40-50years age & 37.5% are from 51-60 years of old. The knowledge regarding glaucoma among adults. Majority 66.55 having poor knowledge, 25% are having good & 8.5 % having excellent knowledge regarding glaucoma . Education have association with knowledge regarding glaucoma at the level of significance at 0.05. Conclusion: Knowledge regarding glaucoma in selected areas of Pune City is found out low. Adults knowledge and Education was found out to be correlated. Recommendation: - 1. A similar study to Descriptive study to assess the attitude regarding glaucoma care among adults can be done. 2. A comparative study of glaucoma & cataract care among elderly people can be done.