Abstract
Background: A vital role of vascular Endothelial Growth Factor was angiogenesis in kidney, that one of the most heavily vascularized organs. the current study aimed to analyze the genotype frequency of VEGF gene polymorphism (-460, -2578 and +936) between 104 hypothyroidism patients at the dialysis unit in Basrah Hospitals and 60 healthy controls, also studied effect on serum level VEGF-A.
Methods: Amplification Refractory Mutation System Real-Time PCR (ARMS RT-PCR) was used to identify the VEGF -460 C/T, -2578C/A, and +936 C/T single nucleotide polymorphisms (SNP) in all included patients.
Results: In comparison to the patient group, the control group had a larger distribution of the VEGF-460 C/T genotype .The CC genotype of -460 SNP showed a higher frequency with the hypothyroidism patients on hemodialysis. For VEGF – 2578 C/A, VEGF + 936 C/T, for cases and healthy control persons, we did not find any differences in genotypes that were statistically significant (p=0.7 and p=0.5, respectively). The dominant model showed a significant association with the protective effect of subclinical hypothyroidism .However ,no significant association was observed between this polymorphism and subclinical hypothyroidism susceptibility in recessive model and allele frequency for -460 C/T. There was no association between -460 rs 833061 polymorphism with overt hypothyroidism in all genetic models .There was no association between VEGF-2578
C/A and +936 C/T polymorphism with hypothyroidism (subclinical ,overt) in all genetic models .VEGF levels in serum from patients were significantly higher than those in healthy subjects .
Conclusion: The VEGF gene is highly polymorphic, and some variations may be connected to ESRD in hypothyroid patients. The pathophysiology of chronic kidney failure may be significantly influenced by VEGF polymorphisms, Our findings indicated that VEGF polymorphisms decreased the risk of hypothyroidism. Since this the first study ,further studies with different races are required to confirm our results.