E-ISSN 2063-5346
 

Original Article 


A PHYSIOLOGICAL STUDY TO SHOW THE EFFECT OF SEMEN FREEZING ON SPERM PARAMETERS AND ITS RELATIONSHIP TO THE SUCCESS RATES OF IVF OPERATIONS

Dhirgam Falih Al-Shimerty, Zainab W. Kermasha, Rana Ahmed Najm, Samer Ali Hasan.

Abstract
The study aimed to try to identify the effect of rapid freezing and thawing processes on the parameters of semen and
sperm, interleukin-10 and the concentration of Almalone Dialdehyde (MDA), in addition to studying the effect of adding types of
antioxidants, which included vitamin C to the sperm freezing medium, in reducing the negative effects resulting from the freezing
processes. Rapid and dissolving in the sperm. The study included the use of Normozoospermic sperm, and the number of samples
was 16 samples of semen samples. The mean age of both sperms was (29.2 ± 1.11 years). The study of the freezing time by
calculating the parameters of the sperm in the sixth month after freezing and thawing compared to before freezing and thawing for
samples of the subjects included in the study showed a significant decrease (P<0.05) in the percentage of sperms with progressive
movement and the percentage of sperm viability and a significant increase ( P<0.05) in the averages of MDA concentration and no
significant differences (P<0.05) in sperm concentration, percentage of normal sperm, circulating cells concentration and
interleukin-10 concentration in the sixth month after freezing and thawing compared to before freezing and thawing. The results of
the study showed that the use of sperm freezing medium (Sperm Freeze) added to the antioxidants of the type C vitamin led to
significant differences (P<0.05) in the studied parameters of the sperm of the subjects included in the study, which led to a
significant (P<0.05) increase in The percentage of sperms with progressive motility and the percentage of sperms viability and a
significant decrease (P<0.05) in the rates of each of the concentrations of Malone Dialdehyde and the absence of significant
differences (P<0.05) in each of the sperm concentration and the percentage of normal sperms and The concentration of circulating
cells and the concentration of interleukin-10 when comparing their rates with the use of sperm freezing medium alone or before
freezing. It is concluded from this study that the processes of rapid freezing and thawing of sperm have negative effects on the
parameters of semen and sperm and the concentration of Malone Dialdehyde (MDA) Which leads to lower pregnancy rates and
IVF technical success, and that the addition of antioxidants of vitamin C type to the medium of freezing sperm (Sperm Freeze)
showed resistance to these negative effects reflected in a significant improvement in studied landmarks.

Key words: IVF, Almalone Dialdehyde (MDA)


 
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How to Cite this Article
Pubmed Style

Dhirgam Falih Al-Shimerty, Zainab W. Kermasha, Rana Ahmed Najm, Samer Ali Hasan. A PHYSIOLOGICAL STUDY TO SHOW THE EFFECT OF SEMEN FREEZING ON SPERM PARAMETERS AND ITS RELATIONSHIP TO THE SUCCESS RATES OF IVF OPERATIONS. ECB. 2022; 11(10): 102-105. doi:10.31838/ecb/2022.11.10.013


Web Style

Dhirgam Falih Al-Shimerty, Zainab W. Kermasha, Rana Ahmed Najm, Samer Ali Hasan. A PHYSIOLOGICAL STUDY TO SHOW THE EFFECT OF SEMEN FREEZING ON SPERM PARAMETERS AND ITS RELATIONSHIP TO THE SUCCESS RATES OF IVF OPERATIONS. https://www.eurchembull.com/?mno=131636 [Access: November 29, 2022]. doi:10.31838/ecb/2022.11.10.013


AMA (American Medical Association) Style

Dhirgam Falih Al-Shimerty, Zainab W. Kermasha, Rana Ahmed Najm, Samer Ali Hasan. A PHYSIOLOGICAL STUDY TO SHOW THE EFFECT OF SEMEN FREEZING ON SPERM PARAMETERS AND ITS RELATIONSHIP TO THE SUCCESS RATES OF IVF OPERATIONS. ECB. 2022; 11(10): 102-105. doi:10.31838/ecb/2022.11.10.013



Vancouver/ICMJE Style

Dhirgam Falih Al-Shimerty, Zainab W. Kermasha, Rana Ahmed Najm, Samer Ali Hasan. A PHYSIOLOGICAL STUDY TO SHOW THE EFFECT OF SEMEN FREEZING ON SPERM PARAMETERS AND ITS RELATIONSHIP TO THE SUCCESS RATES OF IVF OPERATIONS. ECB. (2022), [cited November 29, 2022]; 11(10): 102-105. doi:10.31838/ecb/2022.11.10.013



Harvard Style

Dhirgam Falih Al-Shimerty, Zainab W. Kermasha, Rana Ahmed Najm, Samer Ali Hasan (2022) A PHYSIOLOGICAL STUDY TO SHOW THE EFFECT OF SEMEN FREEZING ON SPERM PARAMETERS AND ITS RELATIONSHIP TO THE SUCCESS RATES OF IVF OPERATIONS. ECB, 11 (10), 102-105. doi:10.31838/ecb/2022.11.10.013



Turabian Style

Dhirgam Falih Al-Shimerty, Zainab W. Kermasha, Rana Ahmed Najm, Samer Ali Hasan. 2022. A PHYSIOLOGICAL STUDY TO SHOW THE EFFECT OF SEMEN FREEZING ON SPERM PARAMETERS AND ITS RELATIONSHIP TO THE SUCCESS RATES OF IVF OPERATIONS. European Chemical Bulletin, 11 (10), 102-105. doi:10.31838/ecb/2022.11.10.013



Chicago Style

Dhirgam Falih Al-Shimerty, Zainab W. Kermasha, Rana Ahmed Najm, Samer Ali Hasan. "A PHYSIOLOGICAL STUDY TO SHOW THE EFFECT OF SEMEN FREEZING ON SPERM PARAMETERS AND ITS RELATIONSHIP TO THE SUCCESS RATES OF IVF OPERATIONS." European Chemical Bulletin 11 (2022), 102-105. doi:10.31838/ecb/2022.11.10.013



MLA (The Modern Language Association) Style

Dhirgam Falih Al-Shimerty, Zainab W. Kermasha, Rana Ahmed Najm, Samer Ali Hasan. "A PHYSIOLOGICAL STUDY TO SHOW THE EFFECT OF SEMEN FREEZING ON SPERM PARAMETERS AND ITS RELATIONSHIP TO THE SUCCESS RATES OF IVF OPERATIONS." European Chemical Bulletin 11.10 (2022), 102-105. Print. doi:10.31838/ecb/2022.11.10.013



APA (American Psychological Association) Style

Dhirgam Falih Al-Shimerty, Zainab W. Kermasha, Rana Ahmed Najm, Samer Ali Hasan (2022) A PHYSIOLOGICAL STUDY TO SHOW THE EFFECT OF SEMEN FREEZING ON SPERM PARAMETERS AND ITS RELATIONSHIP TO THE SUCCESS RATES OF IVF OPERATIONS. European Chemical Bulletin, 11 (10), 102-105. doi:10.31838/ecb/2022.11.10.013