ALTERATIONS IN ADULT MOUSE TESTIS AFTER SUBACUTE INTOXICATION WITH CADMIUM AND MONENSIN DETOXICATION

Ekaterina Pavlova, Juliana Ivanova, Donika Dimova, Yordanka Gluhcheva, Nina Atanassova

Abstract


Cadmium (Cd) is a heavy metal and a major environmental pollutant. The general population is exposed to Cd mainly via drinking water and food. We used a mouse experimental model to investigate the in vivo effects of Cd and the chelating agent Monensin on testis and sperm count in adulthood. Animals were divided into three groups: normal control (receiving distilled water and food): Cd group, exposed to 20 mg/kg b.w. Cd(II) acetate for the first 2 weeks of the experimental protocol and Cd+Monensin group, receiving Monensin (18 mg/kg) after Cd-intoxication (from 15th to 28th day). Histological observations of the testis demonstrated that Cd induced desquamation of germ cell and their assemblance in the luminal region of the tubules. Areas in the testis without spermatides in latest steps of differentiation were also observed in this group. Monensin administration to Cd-treated animals restored histology of the testis to normal in a great extent (despite some Sertoli-cell-only tubules). Statistically significant changes in sperm count were not established for any of the experimental groups. In addition to depleting Cd-concentrations in other investigated organs of Cd-intoxicated mice (our previous data) Monensin can reduce injury of the testis and normalize its morphology after subactute exposure to Cd.  The results, presented in this study demonstrated that Monensin is a good candidate in chelating therapy of some heavy metal intoxications.


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DOI: http://dx.doi.org/10.17628/ecb.2012.1.463-465

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