EFFECTS OF SELENIUM AND SIMULTANEOUS EXPOSURE TO SELENIUM AND DIAZINON ON FEMORAL BONE STRUCTURE IN ADULT RATS
The present study aimed to investigate the macroscopic and microscopic structure of femoral bone tissue in adult male rats after selenium (Se) and simultaneous exposure to Se and Diazinon (DZN). 1month-old male Wistar rats were randomly divided into three groups, each containing 10 males. In the first group (EG1), rats were administered by Se at the dose of 5 mg of Na2SeO3/l in drinking water for 90 days. In the second group (EG2), animals received a drinking water containing 5 mg of Na2SeO3/l and 40 mg of DZN/l for the same treatment period. Ten 1month-old males without Se and DZN administration served as a control group (CG). Our results revealed a significant decrease in femoral length and cortical bone thickness in experimental groups (EG1, EG2) of rats compared to the control ones (CG). Rats from experimental groups (EG1, EG2) also displayed different microstructure in the middle part of the femur, where vascular canals expanded into central area of the bone while, in control rats, these occurred near endosteal surfaces only. Additionally, a smaller number of primary and secondary osteons was identified in experimental groups of rats. A few resorption lacunae were observed in rats simultaneously administered to Se and DZN. Based on these findings we can conclude that subchronic exposure to Se and simultaneous exposure to Se and DZN influences significantly macroscopic and microscopic structure of femoral bone tissue in adult male rats.
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